The Phospho-p53 (S6) Rabbit Polyclonal Antibody (CABP0951) is a valuable tool for researchers studying the phosphorylation of p53 protein, a key player in cell cycle regulation and tumor suppression. This antibody, raised in rabbits, is specifically designed for use in Western blot applications and is highly reactive with human samples.The phosphorylation of p53 at the S6 site is known to play a crucial role in regulating p53 activity and stability, impacting its ability to induce cell cycle arrest or apoptosis in response to cellular stress. The Phospho-p53 (S6) Rabbit Polyclonal Antibody enables precise detection and quantification of phosphorylated p53 at this important site, providing researchers with valuable insights into the mechanisms underlying p53 function in various cell types.
With its high specificity and sensitivity, this antibody is an essential tool for studies in cancer research, as dysregulation of p53 signaling is often associated with tumorigenesis and tumor progression. Furthermore, understanding the phosphorylation status of p53 at the S6 site may offer unique therapeutic opportunities for targeting p53 in cancer treatment.Overall, the Phospho-p53 (S6) Rabbit Polyclonal Antibody (CABP0951) is a reliable and effective reagent for investigating the role of p53 phosphorylation in cell signaling pathways, making it an invaluable asset for researchers exploring the complexities of cancer biology and potential therapeutic strategies.
Product Name:
Phospho-p53-S6 Rabbit Polyclonal Antibody
SKU:
CABP0951
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S6 of human p53 (NP_000537.3).
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method:
Affinity purification
Gene ID:
7157
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from MCF7 cells, using Phospho-p53-S6 Rabbit pAb (CABP0951) at 1:1000 dilution.MCF7 cells were treated by ATP(5 mM) at 30℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
