The Phospho-P53 (S392) Rabbit Monoclonal Antibody (CABP1137) is a high-quality antibody designed for research involving phosphorylated p53 protein at serine 392 residue. P53 is a well-known tumor suppressor protein that plays a crucial role in cell cycle regulation and DNA repair. Phosphorylation of p53 at serine 392 is known to influence its activity and stability, making this antibody essential for studying the functional regulation of p53 in cancer and other disease contexts.This antibody, produced in rabbits, exhibits high specificity and sensitivity towards phospho-p53 (S392) in human samples, making it suitable for use in various applications such as Western blotting and immunohistochemistry.
Its ability to detect and quantify phospho-p53 (S392) protein levels in different cell types enables researchers to explore the role of p53 phosphorylation in cellular processes and disease progression.The phosphorylation status of p53 at serine 392 has been linked to p53-mediated apoptotic responses, DNA damage repair, and cellular stress signaling pathways. Therefore, the use of this antibody in research can provide valuable insights into the mechanisms underlying p53 regulation and its impact on cell fate decisions. This antibody is a valuable tool for researchers studying cancer biology, molecular signaling pathways, and drug development in the field of oncology.
Product Name:
Phospho-p53-S392 Rabbit Monoclonal Antibody
SKU:
CABP1137
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
A synthetic phosphorylated peptide around S392 of human p53 (P04637).
Sequence:
PDSD
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
P53; BCC7; LFS1; BMFS5; TRP53; Phospho-p53-S392
Positive Sample:
C6, C6+Paclitaxel
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Calculated MW:
44kDa
Observed MW:
53kDa
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method:
Affinity purification
Gene ID:
7157
Clone Number:
ARC0309
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of C6 cells, using Phospho-p53-S392 (CABP1137) at 1:1000 dilution.C6 cells were treated by Paclitaxel (100 nM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
