The Phospho-p53 (S376) Rabbit Monoclonal Antibody (CABP0987) is a powerful tool for researchers studying the p53 protein, a key player in cell cycle regulation and apoptosis. This antibody, produced in rabbits, is highly specific for detecting phosphorylation of p53 at serine 376 in human samples, making it an essential tool for studies involving cancer biology and DNA damage response.Phosphorylation of p53 at serine 376 has been linked to the activation of p53-dependent pathways that control cell fate decisions in response to cellular stress. The ability of this antibody to specifically bind to phospho-p53 (S376) allows for precise detection and quantification of this critical post-translational modification in various cell types, providing valuable insights into the mechanisms regulating p53 function.
Research involving phospho-p53 (S376) is essential for understanding the intricate network of signaling pathways that govern cell survival and proliferation, with implications for cancer therapy and drug development. This antibody's high reactivity and specificity make it a valuable tool for investigating the role of phosphorylated p53 in cellular processes and disease progression, offering new avenues for targeted therapeutic strategies.
Product Name:
Phospho-p53-S376 Rabbit Monoclonal Antibody
SKU:
CABP0987
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic phosphorylated peptide around S376 of human p53 (P04637).
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method:
Affinity purification
Gene ID:
7157
Clone Number:
ARC1531
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of 293T cells, using Phospho-p53-S376 Rabbit mAb (CABP0987) at 1:1000 dilution.293T cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
