The Phospho-p53 (S33) Rabbit Monoclonal Antibody (CABP0984) is a specialized tool for researchers studying the phosphorylation of the p53 protein at serine 33, a key post-translational modification involved in regulating cell cycle arrest and apoptosis. This antibody, produced in rabbits, exhibits high specificity for human samples and has been rigorously validated for Western blot applications.The phosphorylation of p53 at serine 33 is known to influence its stability, DNA-binding activity, and transcriptional regulation, ultimately impacting important cellular processes such as DNA repair and cell death pathways. By targeting this specific phosphorylation site, researchers can gain insights into the functional role of p53 in response to cellular stress, DNA damage, and oncogenic signals.
With its ability to detect and quantify phosphorylated p53 at serine 33 in various cell types and experimental conditions, the Phospho-p53 (S33) Rabbit Monoclonal Antibody is an invaluable tool for investigations in cancer biology, genotoxic stress response, and molecular mechanisms underlying p53-mediated tumor suppression. Scientists can use this antibody to uncover novel regulatory networks and potential therapeutic targets associated with p53 signaling pathways.
Product Name:
Phospho-p53-S33 Rabbit Monoclonal Antibody
SKU:
CABP0984
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S33 of human Phospho-p53 (S33) (NP_000537.3).
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method:
Affinity purification
Gene ID:
7157
Clone Number:
ARC1528
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of HT-29 cells, using Phospho-p53-S33 Rabbit mAb (CABP0984) at 1:1000 dilution.HT-29 cells were treated by nocodazole (100 ng/mL) at 37℃ for 16 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
