The Phospho-p53 (S15) Rabbit Polyclonal Antibody (CABP0950) is a valuable tool for researchers studying p53, a tumor suppressor protein involved in cell cycle regulation and DNA repair. This antibody, generated in rabbits, is highly specific to phosphorylated p53 at serine 15 and is validated for use in Western blot and immunohistochemistry applications.Phosphorylation of p53 at serine 15 is known to play a role in regulating p53 protein stability and activity, particularly in response to DNA damage and cellular stress. Detection of phosphorylated p53 at serine 15 is essential for understanding the cellular mechanisms involved in p53-mediated tumor suppression and apoptosis.
This antibody provides researchers with a reliable tool for studying the phosphorylation status of p53 in different cell types and experimental conditions, making it ideal for investigations in cancer biology, genotoxic stress responses, and cell cycle regulation. Its high specificity and sensitivity ensure accurate and reproducible results, allowing for in-depth analysis of p53 signaling pathways and potential therapeutic targets in cancer research.
Product Name:
Phospho-p53-S15 Rabbit Polyclonal Antibody
SKU:
CABP0950
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around S15 of human p53 (NP_000537.3).
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method:
Affinity purification
Gene ID:
7157
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells using Phospho-p53-S15 Rabbit pAb (CABP0950) at 1:900 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 60s.
