The Phospho-p53 (S15) Polyclonal Antibody (CABP0083) is a valuable tool for studying the phosphorylation of p53 at serine 15, a critical post-translational modification that regulates the activity of this tumor suppressor protein. This antibody, raised in rabbits, has been validated for use in various applications, including Western blotting, immunohistochemistry, and immunofluorescence.Phosphorylation of p53 at serine 15 is known to enhance its transcriptional activity, leading to the expression of genes involved in cell cycle arrest, DNA repair, and apoptosis. Dysregulation of this process has been implicated in cancer development and progression, making the Phospho-p53 (S15) Polyclonal Antibody an essential tool for researchers studying p53 signaling in cancer biology.
By facilitating the detection and analysis of phosphorylated p53 at serine 15, this antibody enables researchers to gain insights into the molecular mechanisms underlying p53-mediated cellular responses to stress and DNA damage. Its high specificity and sensitivity make it an ideal choice for investigating the role of p53 phosphorylation in cancer biology and exploring its potential as a therapeutic target for cancer treatment.
Product Name:
Phospho-p53-S15 Rabbit Polyclonal Antibody
SKU:
CABP0083
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S15 of human p53 (NP_000537.3).
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method:
Affinity purification
Gene ID:
7157
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Immunofluorescence analysis of HT-29 treated with UV and HT-29 cells using Phospho-p53-S15 Rabbit pAb (CABP0083) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
