The Phospho-p38 MAPK (T180/Y182) Polyclonal Antibody is a valuable tool for researchers studying the p38 MAPK signaling pathway, which plays a key role in various cellular processes such as cell growth, differentiation, and response to stress. This antibody, raised in rabbits, specifically recognizes phosphorylated p38 MAPK at threonine 180 and tyrosine 182 residues and is highly reactive with human samples.Validated for use in Western blot applications, this antibody provides a reliable means of detecting and analyzing phosphorylated p38 MAPK in different cell types. Its specificity for the phosphorylated form of p38 MAPK makes it ideal for research in immunology, cancer biology, and other fields where the p38 MAPK pathway is of interest.
By targeting the phosphorylated form of p38 MAPK, this antibody enables researchers to gain insights into the activation and regulation of this important signaling pathway, leading to a better understanding of its role in disease processes. Its use in research may ultimately contribute to the development of novel therapeutic strategies targeting p38 MAPK for diseases such as cancer, inflammatory disorders, and neurodegenerative conditions.
Product Name:
Phospho-p38 MAPK-T180/Y182 Polyclonal Antibody
SKU:
CABP1310
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T180/Y182 of human MAPK (NP_620581.1).
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Purification Method:
Affinity purification
Gene ID:
1432
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells using Phospho-p38 MAPK-T180/Y182 Rabbit pAb (CABP1310) at 1:500 dilution. NIH/3T3 cells were treated by UV at room temperature for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
