The Phospho p38 MAPK (T180/Y182) Monoclonal Antibody (CABP1502) is specially designed for research involving the phosphorylated form of p38 MAPK, a key signaling molecule involved in cellular responses to stress and inflammation. This antibody, generated using hybridoma technology, exhibits high specificity and sensitivity towards the phosphorylated p38 MAPK protein in human samples, making it ideal for use in Western blot and immunohistochemistry applications.Phosphorylation of p38 MAPK at threonine 180 and tyrosine 182 residues is crucial for its activation and downstream signaling cascades in response to various stimuli, including environmental stresses, cytokines, and growth factors. The Phospho p38 MAPK (T180/Y182) Monoclonal Antibody enables researchers to detect and analyze phosphorylated p38 MAPK levels in different cell types, providing valuable insights into the regulation of cellular processes such as cell proliferation, differentiation, and apoptosis.
Understanding the role of phosphorylated p38 MAPK in cellular signaling pathways is essential for unraveling its involvement in disease pathogenesis, including inflammation-related disorders, cancer, and neurodegenerative diseases. The Phospho p38 MAPK (T180/Y182) Monoclonal Antibody serves as a valuable tool for investigating the functional implications of p38 MAPK phosphorylation and its potential as a therapeutic target in various disease settings.
Product Name:
Phospho-p38 MAPK-T180/Y182 Monoclonal Antibody
SKU:
CABP1502
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T180/Y182 of human MAPK(NP_620581.1).
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Purification Method:
Affinity purification
Gene ID:
1432/5603/6300/5600
Clone Number:
ARC62666
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of 293T, using Phospho-p38 MAPK-T180/Y182 Rabbit mAb (CABP1502) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
