The Phospho-p38 MAPK (T180) Polyclonal Antibody (CABP1434) is a valuable tool for researchers studying the p38 MAPK signaling pathway, which is involved in a wide range of cellular processes including inflammation, cell proliferation, and apoptosis. This antibody, derived from rabbits, is highly specific to phosphorylated p38 MAPK at threonine 180 in human samples and has been validated for use in Western blotting applications.The phosphorylation of p38 MAPK at T180 is a critical event in the activation of this signaling pathway, leading to downstream effects on gene expression and cellular responses.
By targeting this specific phosphorylation site, researchers can gain insights into the regulation and function of p38 MAPK in various biological contexts.The Phospho-p38 MAPK (T180) Polyclonal Antibody (CABP1434) is an excellent tool for studying the role of p38 MAPK in diseases such as cancer, inflammatory disorders, and neurodegenerative diseases. By understanding the phosphorylation status of p38 MAPK at T180, researchers can uncover potential therapeutic targets and pathways for intervention in these complex diseases.
Product Name:
Phospho-p38 MAPK-T180 Polyclonal Antibody
SKU:
CABP1434
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T180 of human MAPK(NP_620581.1).
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Purification Method:
Affinity purification
Gene ID:
1432
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p38 MAPK-T180 Rabbit pAb (CABP1434) at 1:500 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
