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Phospho-Glucocorticoid Receptor-S211 Rabbit Polyclonal Antibody (CABP0759)

SKU:
CABP0759
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
$503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-Glucocorticoid Receptor-S211 Rabbit Polyclonal Antibody
SKU:CABP0759
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic phosphorylated peptide around S211 of human Glucocorticoid Receptor (NP_000167.1).
Sequence:NESP W
Tested Applications:WB ELISA
Recommended Dilution:WB,1:1000 - 1:2000
Synonyms:GR; GCR; GRL; GCCR; GCRST; Phospho-Glucocorticoid Receptor-S211
Positive Sample:NIH/3T3
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Mitochondrion, Nucleus, Nucleus.
Calculated MW:86kDa
Observed MW:95kDa

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175).

Purification Method:Affinity purification
Gene ID:2908
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of NIH/3T3, using Phospho-Glucocorticoid Receptor-S211 Rabbit pAb (CABP0759) at 1:600 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.

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