The Phospho-NPM1 (T199) Polyclonal Antibody (CABP0836) is a vital tool for researchers studying NPM1, a multifunctional nucleolar phosphoprotein involved in various cellular processes. This antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples, making it ideal for Western blot applications. By targeting the T199 phosphorylation site on NPM1, this antibody enables precise detection and analysis of NPM1 protein levels in different cell types.NPM1 plays a crucial role in regulating cell growth, proliferation, and apoptosis, making it a key player in cancer biology and other diseases.
The phosphorylation of NPM1 at the T199 site has been linked to its functional regulation, highlighting the importance of studying this specific modification in research settings. By using the Phospho-NPM1 (T199) Polyclonal Antibody, researchers can gain valuable insights into the role of NPM1 phosphorylation in various cellular pathways and diseases, paving the way for potential therapeutic interventions targeting this molecular pathway.
Product Name:
Phospho-NPM1-T199 Rabbit Polyclonal Antibody
SKU:
CABP0836
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around T199 of human NPM1 (NP_001032827.1).
Sequence:
RDTP A
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
B23; NPM; Phospho-NPM1-T199
Positive Sample:
HeLa+Nocodazole
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, nucleolus, nucleoplasm.
Calculated MW:
33kDa
Observed MW:
38kDa
The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified.
Purification Method:
Affinity purification
Gene ID:
4869
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Phospho-NPM1-T199 Rabbit pAb (CABP0836) at 1:900 dilution.HeLa cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
