The Phospho-NOS3 (S1177) Polyclonal Antibody (CABP0421) is designed for researchers studying NOS3, a key enzyme involved in the production of nitric oxide which plays a critical role in cardiovascular health and function. This antibody, produced in rabbits, is highly specific for the phosphorylated form of NOS3 at Serine 1177, making it ideal for detecting and analyzing phosphorylation events in Western blot experiments.NOS3, also known as endothelial nitric oxide synthase, is essential for regulating blood vessel relaxation and blood flow, and dysfunction in NOS3 activity has been implicated in various cardiovascular diseases such as hypertension and atherosclerosis.
Phosphorylation of NOS3 at Serine 1177 is known to enhance its enzymatic activity, highlighting the importance of studying this post-translational modification in cardiovascular research.By using the Phospho-NOS3 (S1177) Polyclonal Antibody, researchers can gain insights into the regulation and function of NOS3 in cardiovascular physiology and pathology. This antibody is validated for use in Western blot applications with human samples, providing a valuable tool for studies aiming to understand the role of NOS3 phosphorylation in cardiovascular diseases and potential therapeutic interventions targeting this signaling pathway.
Product Name:
Phospho-eNOS-S1177 Rabbit Polyclonal Antibody
SKU:
CABP0421
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S1177 of human eNOS (NP_000594.2).
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
4846
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Phospho-eNOS-S1177 Rabbit pAb (CABP0421).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
