The Phospho-NFKBIA (Y42) Polyclonal Antibody (CABP0420) is a valuable tool for researchers studying the phosphorylation of NFKBIA, a key regulator of the NF-κB pathway involved in inflammation and immune responses. This antibody, raised in rabbits, has been extensively validated for use in Western blot applications and specifically detects phosphorylation of NFKBIA at tyrosine 42.NFKBIA acts as a negative regulator of NF-κB by binding to the p65 subunit and preventing its translocation into the nucleus. Phosphorylation of NFKBIA at tyrosine 42 can modulate its function, leading to altered NF-κB signaling and gene expression. Research on this phosphorylation event is crucial for understanding the intricate regulation of the NF-κB pathway and its implications in various diseases, including cancer, autoimmune disorders, and inflammatory conditions.
With its high reactivity towards human samples and specificity for phosphorylated NFKBIA, the Phospho-NFKBIA (Y42) Polyclonal Antibody (CABP0420) is a valuable tool for investigating the role of NFKBIA phosphorylation in immune regulation and disease states. Its use in Western blotting provides researchers with a reliable method for detecting and analyzing phosphorylated NFKBIA in various cell types, advancing our understanding of NF-κB signaling pathways and potential therapeutic targets.
Product Name:
Phospho-IκBα-Y42 Rabbit Polyclonal Antibody
SKU:
CABP0420
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho specific peptide corresponding to residues surrounding Y42 of human IκBα
Tested Applications:
WB
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
IKBA; MAD-3; NFKBI; EDAID2; Phospho-IκBα-Y42
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
36kDa
Observed MW:
40kDa
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
Purification Method:
Affinity purification
Gene ID:
4792
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cellss, using Phospho-IκBα-Y42 Rabbit pAb (CABP0420).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
