The Phospho-NFkB1 (S337) Polyclonal Antibody (CABP0125) is a valuable tool for researchers studying NFkB1, a key transcription factor involved in regulating immune and inflammatory responses. This antibody, generated in rabbits, is highly specific for detecting phosphorylation at serine 337 of the NFkB1 protein in human samples. Validated for use in Western blot applications, it allows for accurate detection and analysis of NFkB1 phosphorylation levels in various cell types.NFkB1 is a critical player in the immune response, controlling the expression of genes involved in inflammation, cell survival, and immunity.
Phosphorylation of NFkB1 at serine 337 is known to regulate its activity, making it an important site of study for understanding the mechanisms of immune regulation. Research on NFkB1 phosphorylation has implications for diseases such as cancer, autoimmune disorders, and inflammatory conditions, making this antibody a valuable tool for investigating potential therapeutic targets in these areas.
Product Name:
Phospho-NFKB1-S337 Rabbit Polyclonal Antibody
SKU:
CABP0125
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S337 of human NFKB1 (NP_001158884.1).
This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. NFKB is a critical regulator of the immediate-early response to viral infection. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.
Purification Method:
Affinity purification
Gene ID:
4790
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-NFKB1 p105/p50-S337 Rabbit pAb (CABP0125) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
