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Phospho-NF-kB p65/RelA-T505 Polyclonal Antibody (CABP1318)

SKU:
CABP1318
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
$167 - $587
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-NF-kB p65/RelA-T505 Polyclonal Antibody
SKU:CABP1318
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around T505 of human Phospho-NF-kB p65/RelA-T505 (NP_068810.3).
Sequence:LVTG A
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:p65; CMCU; NFKB3; AIF3BL3; Phospho-NF-kB p65/RelA-T505
Positive Sample:U-2 OS+cisplatin
Conjugate:Unconjugated
Cellular Localization:cytoplasm, cytosol, glutamatergic synapse, nucleoplasm, nucleus
Calculated MW:60kDa
Observed MW:65kDa

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:5970
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of U-2 OS, using Phospho-NF-kB p65/RelA-T505 Rabbit pAb (CABP1318) at 1:1000 dilution.U-2 OS cells were treated by cisplatin at 37℃ for 16 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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