null

Phospho-NF-kB p65/RelA-S536 Monoclonal Antibody (CABP1294)

SKU:
CABP1294
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
€139 - €609
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-NF-kB p65/RelA-S536 Monoclonal Antibody
SKU:CABP1294
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S536 of human Phospho-NF-kB p65/RelA-S536 (NP_068810.3).
Sequence:FSSI A
Tested Applications:WB ELISA
Recommended Dilution:WB,1:1000 - 1:5000
Synonyms:p65; CMCU; NFKB3; AIF3BL3; Phospho-NF-kB p65/RelA-S536
Positive Sample:HeLa,NIH/3T3,C6
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:60kDa
Observed MW:65kDa

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:5970
Clone Number:ARC55585
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from HeLa cells, using Phospho-NF-kB p65/RelA-S536 Rabbit mAb (CABP1294) at1:2000 dilution.HeLa cells were treated by100ng/mL TNF-α + 50nM CA at 37℃ for 30 minutes after serum-starvation overnightSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Related Products