The Phospho-NF-kB p65 (RELA) [S536] Monoclonal Antibody from Assay Genie (SKU: AG242) is specifically designed for research involving the phosphorylation of NF-kB p65 (RELA) at serine 536. NF-kB is a transcription factor that plays a crucial role in regulating the expression of genes involved in immune responses, inflammation, cell proliferation, and survival. Phosphorylation of NF-kB p65 at serine 536 is known to activate its transcriptional activity and is implicated in various disease states, including cancer, inflammatory disorders, and autoimmune diseases.This monoclonal antibody, raised in mice, is highly specific for the phosphorylated form of NF-kB p65 (RELA) at serine 536 and demonstrates high sensitivity and specificity in Western blot applications.
It allows for the detection and analysis of phosphorylated NF-kB p65 (RELA) in various cell types and tissues, making it an ideal tool for studies in immunology, cancer research, and inflammatory diseases.Understanding the regulation of NF-kB activity and the role of phosphorylation in modulating its function is essential for elucidating the mechanisms underlying immune responses and inflammation. The Phospho-NF-kB p65 (RELA) [S536] Monoclonal Antibody from Assay Genie provides researchers with a valuable tool for investigating these processes and developing novel therapeutic strategies targeting NF-kB
Product Name:
Phospho-NF-kB p65/RelA-S536 Monoclonal Antibody
SKU:
CABP1294
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S536 of human Phospho-NF-kB p65/RelA-S536 (NP_068810.3).
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5970
Clone Number:
ARC55585
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-NF-kB p65/RelA-S536 Rabbit mAb (CABP1294) at1:2000 dilution.HeLa cells were treated by100ng/mL TNF-α + 50nM CA at 37℃ for 30 minutes after serum-starvation overnightSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
