The Phospho-Nedd4L (S448) Polyclonal Antibody (CABP0843) is a valuable tool for researchers studying Nedd4L, a key protein involved in cell signaling and regulation. This antibody, produced in rabbits, is highly specific to the phosphorylated form of Nedd4L at Serine 448 and has been validated for use in multiple applications, including Western blot and immunohistochemistry.Nedd4L plays a significant role in regulating protein degradation and trafficking, particularly in the context of cell growth, differentiation, and survival. Phosphorylation of Nedd4L at Serine 448 has been implicated in various cellular processes, making this antibody essential for investigating the mechanism of action and functional consequences of this post-translational modification.
Researchers studying signal transduction pathways, protein degradation, and cellular growth and survival will benefit from the high specificity and sensitivity of the Phospho-Nedd4L (S448) Polyclonal Antibody. Its ability to detect phosphorylated Nedd4L in different cell types and tissues makes it a valuable tool for advancing our understanding of molecular mechanisms underlying disease states such as cancer, neurodegenerative disorders, and metabolic diseases.
Product Name:
Phospho-NEDD4L-S448 Rabbit Polyclonal Antibody
SKU:
CABP0843
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S448 of human NEDD4L (NP_001138439.1).
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
23327
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-NEDD4L-S448 Rabbit pAb (CABP0843) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour. C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
