NCF1 Polyclonal Antibody (CAB1148) is a specialized antibody designed for research focusing on NCF1, a cytosolic subunit of the phagocyte NADPH oxidase complex. This antibody, produced from rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the NCF1 protein, researchers can detect and analyze NCF1 levels in various cell types, making it a valuable tool for studies in immunology and related fields.NCF1, also known as neutrophil cytosolic factor 1, plays a crucial role in the production of reactive oxygen species in phagocytes, which are essential for microbial killing.
Dysfunction of NCF1 has been associated with autoimmune disorders and chronic inflammatory conditions, making it a potential therapeutic target for research in diseases such as rheumatoid arthritis and systemic lupus erythematosus. Understanding the function of NCF1 through the use of this antibody is essential for advancing our knowledge of immune responses and developing targeted treatments for these conditions.
Product Name:
p47phox/NCF1 Rabbit Polyclonal Antibody
SKU:
CAB1148
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-390 of human p47phox/NCF1 (NP_000256.4).
The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.
Purification Method:
Affinity purification
Gene ID:
653361
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using p47phox/NCF1 Rabbit pAb (CAB1148) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
