The NBN Polyclonal Antibody (CAB0783) is a valuable tool for research involving the NBN protein, which is important for maintaining genome stability and DNA repair processes. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.The NBN protein, also known as nibrin, is part of the MRN complex involved in DNA double-strand break repair. Mutations in the NBN gene have been associated with a predisposition to certain types of cancer, making it a promising target for cancer research.
By detecting and analyzing NBN protein levels in various cell types, this antibody can aid in studying DNA damage response pathways and potential therapeutic strategies for cancer treatment.Researchers interested in understanding the mechanisms of genome stability, DNA repair, and cancer development will find the NBN Polyclonal Antibody (CAB0783) to be a valuable asset in their studies. Its high specificity and reactivity with human samples make it a reliable resource for investigating the role of NBN in cellular processes and disease pathways.
Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.
Purification Method:
Affinity purification
Gene ID:
4683
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and NBS1/NBS1/NBN knockout (KO) 293T cells, using [KO Validated] NBS1/NBN Rabbit pAb (CAB0783) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
