The Phospho-NaK-TBK1 (S172) Rabbit Monoclonal Antibody (CABP1026) is a cutting-edge tool for researchers studying phosphorylation events in the NaK-TBK1 pathway. This antibody, developed using advanced monoclonal technology, specifically targets the phosphorylated form of NaK-TBK1 at serine 172. It is highly sensitive and selective, making it ideal for detecting and studying this important signaling pathway.NaK-TBK1 is a key regulator of innate immune responses and is involved in various cellular processes, including inflammation, autophagy, and antiviral responses. Phosphorylation of NaK-TBK1 at serine 172 is a crucial modification that activates its kinase activity, leading to downstream signaling cascades.
By using this antibody, researchers can investigate the role of NaK-TBK1 phosphorylation in various biological processes and diseases, such as viral infections, autoimmune disorders, and cancer.In addition to its high specificity and sensitivity, the Phospho-NaK-TBK1 (S172) Rabbit Monoclonal Antibody (CABP1026) has been rigorously validated for use in Western blot applications, ensuring reliable and reproducible results. It is compatible with human samples and various cell types, making it a versatile tool for immunology and cancer research. Researchers can rely on this antibody to accurately detect and analyze phosphorylated NaK-TBK1, furthering our understanding
Product Name:
Phospho-TBK1/NAK-S172 Rabbit Monoclonal Antibody
SKU:
CABP1026
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around S172 of human TBK1/NAKTBK1 (Q9UHD2).
Sequence:
FVSL Y
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
NAK; T2K; IIAE8; FTDALS4; Phospho-TBK1/NAK-S172
Positive Sample:
HeLa CA,NIH/3T3 CA
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
84kDa
Observed MW:
84kDa
The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. The protein is also an important kinase for antiviral innate immunity response.
Purification Method:
Affinity purification
Gene ID:
29110
Clone Number:
ARC1571
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05%BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-TBK1/NAK-S172 Rabbit mAb (CABP1026) at 1:1000 dilution.Both HeLa cells and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1min.
