The Phospho Na+/K+ ATPase (S16) Rabbit Polyclonal Antibody (CABP1065) is a crucial tool for researchers studying the phosphorylation of the Na+/K+ ATPase protein. This antibody, specifically designed for detecting the phosphorylation of the S16 residue of the Na+/K+ ATPase, is raised in rabbits and is highly reactive with human samples. The Na+/K+ ATPase enzyme plays a vital role in maintaining the electrochemical gradient across cell membranes, contributing to processes such as nerve conduction, muscle contraction, and cell signaling. Phosphorylation of the S16 residue of the Na+/K+ ATPase is known to regulate its activity and is essential for its proper function.
This antibody is validated for use in Western blot applications, enabling researchers to detect and analyze the phosphorylated form of the Na+/K+ ATPase in various cell types. Understanding the phosphorylation of the Na+/K+ ATPase at the S16 residue can provide insights into cellular signaling pathways and the regulation of ion transport processes.Researchers in the fields of cell biology, neuroscience, and cardiovascular research can benefit from using the Phospho Na+/K+ ATPase (S16) Rabbit Polyclonal Antibody (CABP1065) to further their understanding of cellular function and signaling mechanisms.
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
476
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Phospho-Na+/K+-ATPase-S16 Rabbit pAb (CABP1065) at 1:1000 dilution.293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
