The Phospho-Myosin Light Chain 2 (T18/S19) Monoclonal Antibody is a crucial tool for researchers studying cellular processes involving the myosin light chain 2 protein. This antibody, produced through a monoclonal production process, shows high specificity and sensitivity towards the phosphorylated form of myosin light chain 2 at threonine 18 and serine 19 residues.Myosin light chain 2 is a key regulator of actin-myosin interactions in muscle contraction and cell motility. Phosphorylation of myosin light chain 2 at T18/S19 is known to play a role in various cellular processes, including smooth muscle contraction, cell migration, and cytokinesis.
This antibody specifically targets the phosphorylated form of myosin light chain 2, allowing researchers to study and analyze its role in these processes.With its reliable performance in Western blot applications and reactivity towards human samples, the Phospho-Myosin Light Chain 2 (T18/S19) Monoclonal Antibody is a valuable tool for researchers in cell biology, physiology, and molecular biology. By enabling the detection and analysis of phosphorylated myosin light chain 2, this antibody contributes to a better understanding of the mechanisms underlying cellular functions and diseases related to myosin regulation.
cell cortex, cytoplasm, cytosol, myofibril, Z disc
Calculated MW:
20kDa
Observed MW:
18kDa
Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
10398
Clone Number:
ARC62066
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of HeLa, using Phospho-Myosin Light Chain 2-T18/S19 Rabbit mAb (CABP1503) at 1:6000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
