The Phospho-Myosin Light Chain 2 (S19) Monoclonal Antibody is a specialized tool for researchers studying phosphorylated myosin light chain 2, a key regulatory protein involved in smooth muscle contraction and cell motility. This antibody, produced by Assay Genie, specifically targets the phosphorylation site at serine 19 of myosin light chain 2, allowing for precise detection and analysis of this post-translational modification in various experimental systems.This monoclonal antibody is highly sensitive and specific to phosphorylated myosin light chain 2 at serine 19, making it an essential tool for investigating the role of this protein in cellular processes such as muscle contraction, cell migration, and cytoskeletal dynamics.
It has been rigorously validated for use in Western blotting, immunofluorescence, and immunohistochemistry applications, providing researchers with reliable and reproducible results in their experiments.By using the Phospho-Myosin Light Chain 2 (S19) Monoclonal Antibody, researchers can gain valuable insights into the regulation of smooth muscle function, cell motility, and disease processes such as cancer metastasis and cardiovascular disorders. This antibody holds great potential for advancing our understanding of cellular physiology and pathology, making it an indispensable tool for scientists studying signaling pathways and molecular mechanisms involving phosphorylated myosin light chain 2.
cell cortex, cytoplasm, cytosol, myofibril, Z disc
Calculated MW:
20kDa
Observed MW:
18kDa
Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
10398
Clone Number:
ARC62046
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of HeLa, using Phospho-Myosin Light Chain 2-S19 Rabbit mAb (CABP1433) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
