The Phospho-MYL9 (T18/S19) Rabbit Polyclonal Antibody (CABP0955) is a valuable tool for researchers studying MYL9, a key protein involved in the regulation of cell motility and contraction. This antibody, generated in rabbits, is highly specific for detecting phosphorylation at threonine 18 and serine 19 residues of MYL9 in human samples. Validated for use in Western blot applications, it enables the detection and analysis of MYL9 phosphorylation levels in various cell types.MYL9, also known as myosin regulatory light chain 9, plays a crucial role in the control of actin-myosin interactions and cell contractility. Phosphorylation of MYL9 at threonine 18 and serine 19 sites is known to regulate its activity and impact cellular processes such as cell migration, adhesion, and cytokinesis.
Understanding the phosphorylation status of MYL9 is essential for unraveling its role in physiological and pathological conditions, including cancer metastasis and smooth muscle disorders.By using the Phospho-MYL9 (T18/S19) Rabbit Polyclonal Antibody (CABP0955), researchers can gain insights into the molecular mechanisms underlying MYL9 function and its potential implications in various diseases. This antibody serves as a powerful tool for investigating the signaling pathways and biological processes mediated by MYL9 phosphorylation, paving the way for the development of targeted
Product Name:
Phospho-MYL9-T18/S19 Rabbit Polyclonal Antibody
SKU:
CABP0955
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T18 & S19 of human MYL9 (NP_006088.2).
cell cortex, cytoplasm, cytosol, myofibril, Z disc
Calculated MW:
20kDa
Observed MW:
18kDa
Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
10398
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-MYL9-T18/S19 Rabbit pAb (CABP0955) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
