The Phospho-MyL9 (S19) Polyclonal Antibody (CABP0872) is a valuable tool for researchers studying the role of MyL9 in various cellular processes. Myosin regulatory light chain 9 (MyL9) is a key regulatory protein involved in actomyosin contractility and cell motility. This antibody specifically recognizes phosphorylated MyL9 at serine 19, enabling the detection and analysis of phosphorylated MyL9 in a variety of cell types.Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. By targeting the phosphorylated form of MyL9, researchers can investigate the regulation of actomyosin contractility in different cellular contexts.
This antibody is an essential tool for studies in cell biology, cancer research, and cardiovascular disease, where MyL9 phosphorylation plays a crucial role in cellular function and disease progression.Understanding the phosphorylation of MyL9 at serine 19 is essential for unraveling the complex signaling pathways that control cell motility and contractility. By using this antibody, researchers can gain valuable insights into the molecular mechanisms underlying these processes and develop potential therapeutic strategies targeting MyL9 phosphorylation for the treatment of various diseases.
Product Name:
Phospho-MYL9-S19 Rabbit Polyclonal Antibody
SKU:
CABP0872
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S19 of human MYL9 (NP_006088.2).
cell cortex, cytoplasm, cytosol, myofibril, Z disc
Calculated MW:
20kDa
Observed MW:
18kDa
Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
10398
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of C2C12, using Phospho-MYL9-S19 Rabbit pAb (CABP0872) at 1:400 dilution.C2C12 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
