The Phospho-Myc (T58) Polyclonal Antibody (CABP0080) is a valuable tool for researchers studying the phosphorylation of the Myc protein at threonine 58. This post-translational modification plays a key role in regulating Myc protein stability, transcriptional activity, and ultimately cell proliferation.Raised in rabbits, this antibody is highly specific for human samples and has been validated for use in Western blot applications, allowing for precise detection and quantification of phospho-Myc (T58) levels in various cell types. Its high reactivity and specificity make it an ideal choice for studies focused on understanding the signaling pathways involving Myc protein and its role in cancer progression.The phosphorylation of Myc at threonine 58 has been linked to the dysregulation of cell growth and survival in numerous cancer types, making it a promising target for therapeutic intervention.
By using this antibody to study the dynamics of Myc phosphorylation, researchers can gain valuable insights into the mechanisms driving oncogenic processes and potentially identify novel targets for anti-cancer therapies.Overall, the Phospho-Myc (T58) Polyclonal Antibody (CABP0080) represents a powerful tool for investigating the intricate regulatory pathways governing Myc protein function and its implications in cancer biology. Its specificity, reliability, and versatility make it an essential component of any research program focused on understanding the molecular mechanisms underlying cancer development and progression.
Product Name:
Phospho-c-Myc-T58 Rabbit Polyclonal Antibody
SKU:
CABP0080
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T58 of mouse MYC (NP_001170823.1).
Sequence:
LPTP P
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:100 - 1:500 IHC-P,1:50 - 1:200
Synonyms:
MRTL; MYCC; c-Myc; bHLHe39; Phospho-c-Myc-T58
Positive Sample:
293T
Conjugate:
Unconjugated
Cellular Localization:
Nucleus, nucleolus, nucleoplasm.
Calculated MW:
51kDa
Observed MW:
62kDa
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
Purification Method:
Affinity purification
Gene ID:
4609
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Phospho-c-Myc-T58 Rabbit pAb (CABP0080) at 1:500 dilution.293T cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
