The Phospho-Myc (T358) Polyclonal Antibody (CABP0411) is a valuable tool for researchers studying the phosphorylation of the Myc protein. Myc is a critical regulator of cell growth and proliferation, and its phosphorylation at threonine 358 is known to modulate its activity. This antibody, produced in rabbits, specifically recognizes the phosphorylated form of Myc at T358, allowing for precise detection and analysis in various experimental settings.Validated for use in Western blot applications, the Phospho-Myc (T358) Polyclonal Antibody is highly reactive with human samples, making it an ideal choice for researchers studying signaling pathways involving Myc in cancer, cell cycle regulation, and other biological processes.
By targeting the phosphorylated form of Myc, this antibody provides researchers with a powerful tool for understanding the role of Myc phosphorylation in cellular signaling and disease pathways.
Product Name:
Phospho-c-Myc-T358 Rabbit Polyclonal Antibody
SKU:
CABP0411
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho specific peptide corresponding to residues surrounding T358 of human c-Myc
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
MRTL; MYCC; c-Myc; bHLHe39; Phospho-c-Myc-T358
Positive Sample:
HT-29
Conjugate:
Unconjugated
Cellular Localization:
Nucleus, nucleolus, nucleoplasm.
Calculated MW:
51kDa
Observed MW:
65kDa
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
Purification Method:
Affinity purification
Gene ID:
4609
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HT29 cells untreated or treated with UV, using Phospho-c-Myc-T358 Rabbit pAb (CABP0411).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
