The Phospho-Myc (S62) Polyclonal Antibody (CABP0082) is designed for research involving the phosphorylated Myc protein, a key regulator of cell growth, proliferation, and apoptosis. The antibody, raised in rabbits, is highly specific to phosphorylated Myc at serine 62 and is validated for use in Western blot and immunohistochemistry applications. It enables precise detection and quantification of phosphorylated Myc in various cell types, making it an essential tool for studies in cancer biology and signaling pathways.Phosphorylated Myc at serine 62 plays a crucial role in regulating Myc protein stability, transcriptional activity, and oncogenic potential.
Dysregulation of phosphorylation at this site has been implicated in various cancers, making it a potential therapeutic target for cancer treatment. Understanding the dynamics of phosphorylated Myc at serine 62 is essential for developing targeted therapies and personalized medicine approaches for cancer patients.Overall, the Phospho-Myc (S62) Polyclonal Antibody (CABP0082) is a valuable tool for researchers studying Myc signaling in cancer and other diseases, providing insights into the role of phosphorylation in Myc-mediated oncogenesis and offering potential targets for therapeutic intervention.
Product Name:
Phospho-c-Myc-S62 Rabbit Polyclonal Antibody
SKU:
CABP0082
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A phospho specific peptide corresponding to residues surrounding S62 of human MYC (NP_001341799.1).
Sequence:
PLSP SR
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:
MRTL; MYCC; c-Myc; bHLHe39; Phospho-c-Myc-S62
Positive Sample:
NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
Nucleus, nucleolus, nucleoplasm.
Calculated MW:
51kDa
Observed MW:
62kDa
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
Purification Method:
Affinity purification
Gene ID:
4609
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-c-Myc-S62 Rabbit pAb (CABP0082) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
