The Phospho-Myc S373 Polyclonal Antibody (CABP0132) is specifically designed for research exploring the phosphorylation of the Myc protein at serine 373. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. It targets the phosphorylated form of the Myc protein, allowing for precise detection and analysis in a variety of cell types, making it an excellent tool for investigations in signal transduction pathways and cancer research.The phosphorylation of Myc at serine 373 is known to play a pivotal role in regulating the activity and stability of the Myc protein, which is a key player in cell growth, proliferation, and apoptosis.
Understanding the regulation of Myc phosphorylation at this specific site is crucial for unraveling its role in cancer development and progression. This antibody provides researchers with a valuable tool to delve deeper into the intricate mechanisms underlying Myc signaling and its implications in cancer biology.
Product Name:
Phospho-c-Myc-S373 Rabbit Polyclonal Antibody
SKU:
CABP0132
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho specific peptide corresponding to residues surrounding S373 of human c-Myc
Tested Applications:
WB
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
MRTL; MYCC; c-Myc; bHLHe39; Phospho-c-Myc-S373
Positive Sample:
A-431
Conjugate:
Unconjugated
Cellular Localization:
Nucleus, nucleolus, nucleoplasm.
Calculated MW:
51kDa
Observed MW:
60kDa
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
Purification Method:
Affinity purification
Gene ID:
4609
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from A431 cells using Phospho-c-Myc-S373 Rabbit pAb (CABP0132).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
