The Phospho-mTOR (S2481) Rabbit Polyclonal Antibody (CABP0490) is a valuable tool for researchers studying the mTOR signaling pathway, a key regulator of cell growth, proliferation, and metabolism. This antibody is raised in rabbits and specifically detects phosphorylation of mTOR at serine 2481 in human samples. It has been validated for use in Western blot applications, allowing for the precise detection and analysis of phospho-mTOR in various cell types.mTOR (mechanistic target of rapamycin) is a central player in cellular processes such as protein synthesis, autophagy, and cell survival, making it a crucial target for research in cancer, metabolic disorders, and neurodegenerative diseases.
The phosphorylation of mTOR at serine 2481 is associated with its activation and downstream signaling, making this antibody an essential tool for studying the regulation of mTOR activity in different physiological and pathological conditions.By using the Phospho-mTOR (S2481) Rabbit Polyclonal Antibody (CABP0490), researchers can gain valuable insights into the intricate mechanisms of mTOR signaling and its role in various diseases, paving the way for the development of targeted therapies to modulate mTOR activity for therapeutic purposes.
Product Name:
Phospho-mTOR-S2481 Rabbit Polyclonal Antibody
SKU:
CABP0490
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S2481 of human mTOR (NP_004949.1).
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This kinase is a component of two distinct complexes, mTORC1, which controls protein synthesis, cell growth and proliferation, and mTORC2, which is a regulator of the actin cytoskeleton, and promotes cell survival and cell cycle progression. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Inhibitors of mTOR are used in organ transplants as immunosuppressants, and are being evaluated for their therapeutic potential in SARS-CoV-2 infections. Mutations in this gene are associated with Smith-Kingsmore syndrome and somatic focal cortical dysplasia type II. The ANGPTL7 gene is located in an intron of this gene.
Purification Method:
Affinity purification
Gene ID:
2475
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells using Phospho-mTOR-S2481 Rabbit pAb (CABP0490) at 1:2000 dilution. C6 cells were treated by IGF-1 (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 30s.
