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Phospho-mTOR-S2481 Rabbit Polyclonal Antibody (CABP0490)

SKU:
CABP0490
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-mTOR-S2481 Rabbit Polyclonal Antibody
SKU:CABP0490
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S2481 of human mTOR (NP_004949.1).
Sequence:IHSF I
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; Phospho-mTOR-S2481
Positive Sample:C6
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Cytoplasmic side, Endoplasmic reticulum membrane, Golgi apparatus membrane, Lysosome, Mitochondrion outer membrane, Nucleus, PML body, Peripheral membrane protein.
Calculated MW:289kDa
Observed MW:289kDa

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This kinase is a component of two distinct complexes, mTORC1, which controls protein synthesis, cell growth and proliferation, and mTORC2, which is a regulator of the actin cytoskeleton, and promotes cell survival and cell cycle progression. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Inhibitors of mTOR are used in organ transplants as immunosuppressants, and are being evaluated for their therapeutic potential in SARS-CoV-2 infections. Mutations in this gene are associated with Smith-Kingsmore syndrome and somatic focal cortical dysplasia type II. The ANGPTL7 gene is located in an intron of this gene.

Purification Method:Affinity purification
Gene ID:2475
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from C6 cells using Phospho-mTOR-S2481 Rabbit pAb (CABP0490) at 1:2000 dilution. C6 cells were treated by IGF-1 (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 30s.

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