The Phospho-mTOR (S2448) Rabbit Monoclonal Antibody (CABP0115) is a valuable tool for researchers studying the mTOR signaling pathway. This antibody, generated from rabbit monoclonal cells, is highly specific to the phosphorylated form of mTOR at serine 2448 in human samples. It has been validated for use in Western blot applications, allowing for the detection and analysis of phospho-mTOR in various cell types.mTOR, a key regulator of cell growth and metabolism, is involved in various cellular processes such as protein synthesis, autophagy, and cell proliferation. Phosphorylation of mTOR at serine 2448 is known to activate its kinase activity, leading to downstream signaling that influences cell survival and growth.
Understanding the phosphorylation status of mTOR is essential for elucidating its role in cancer biology, metabolism, and cellular homeostasis.Researchers in the fields of cancer biology, cell signaling, and metabolic disorders can benefit from using the Phospho-mTOR (S2448) Rabbit Monoclonal Antibody in their studies. By targeting the phosphorylated form of mTOR, this antibody provides a specific and reliable tool for investigating the complex functions of mTOR in health and disease.
Product Name:
Phospho-mTOR-S2448 Rabbit Monoclonal Antibody
SKU:
CABP0115
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S2448 of human mTOR (P42345).
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This kinase is a component of two distinct complexes, mTORC1, which controls protein synthesis, cell growth and proliferation, and mTORC2, which is a regulator of the actin cytoskeleton, and promotes cell survival and cell cycle progression. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Inhibitors of mTOR are used in organ transplants as immunosuppressants, and are being evaluated for their therapeutic potential in SARS-CoV-2 infections. Mutations in this gene are associated with Smith-Kingsmore syndrome and somatic focal cortical dysplasia type II. The ANGPTL7 gene is located in an intron of this gene.
Purification Method:
Affinity purification
Gene ID:
2475
Clone Number:
ARC0094
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of 293T cells, using Phospho-mTOR-S2448 Rabbit mAb (CABP0115) at 1:1000 dilution.293T cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight or treated by IGF-1 (50 ng/mL) at 37℃ for 5 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
