The Phospho-MST1/STK4 (T387) Rabbit Polyclonal Antibody (CABP0906) is a valuable tool for researchers studying the phosphorylation of MST1/STK4 at threonine 387. This antibody, raised in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the phosphorylated form of MST1/STK4, this antibody allows for the detection and analysis of this critical kinase in various cell types.MST1/STK4 plays a key role in cell signaling pathways involved in regulating cell growth, apoptosis, and immunity. Phosphorylation at threonine 387 is known to modulate the activity of MST1/STK4, impacting its functions in these pathways.
Research into the phosphorylation status of MST1/STK4 is essential for understanding its role in diseases such as cancer, autoimmune disorders, and inflammatory conditions.By using the Phospho-MST1/STK4 (T387) Rabbit Polyclonal Antibody (CABP0906), researchers can delve deeper into the mechanisms by which MST1/STK4 contributes to disease pathogenesis, potentially leading to the development of targeted therapies for these conditions. This antibody is a valuable tool for studies in cancer biology, immunology, and signal transduction research.
Product Name:
Phospho-MST1/STK4-T387 Rabbit Polyclonal Antibody
SKU:
CABP0906
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T387 of human MST1/STK4 (NP_006273.1).
Sequence:
DETM Q
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
KRS2; MST1; YSK3; Phospho-MST1/STK4-T387
Positive Sample:
NIH/3T3,C6
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
56kDa
Observed MW:
59kDa
The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.
Purification Method:
Affinity purification
Gene ID:
6789
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 and C6 cells, using phospho-MST1/STK4-T387 pAb (CABP0906) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
