The Phospho-MRE11 (Ser676) Polyclonal Antibody is a specialized research tool designed for the study of MRE11, a key protein involved in DNA repair and genomic stability. This antibody, produced in rabbits, is highly specific for human samples and is optimized for use in Western blot applications. It binds specifically to the phosphorylated form of MRE11 at serine 676, allowing for precise detection and analysis in various cell types.MRE11 is a crucial component of the MRN complex, which plays a central role in the detection and repair of DNA double-strand breaks. Phosphorylation of MRE11 at serine 676 is known to regulate its function in DNA repair pathways, making this antibody a valuable tool for studying the mechanisms of genomic stability and DNA damage response.
Researchers studying DNA repair, genomic instability, and the cellular response to DNA damage will find this Phospho-MRE11 (Ser676) Polyclonal Antibody to be a valuable asset in their experimental toolbox. Its specificity, sensitivity, and reliability make it an ideal choice for investigations into the molecular mechanisms underlying these critical biological processes.
Product Name:
Phospho-MRE11 (Ser676) Polyclonal Antibody
SKU:
CABP1514
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S676 of human MRE11 (NP_005582.1).
This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
4361
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells using Phospho-MRE11 (Ser676) Rabbit pAb (CABP1514) at 1:800 dilution. HeLa cells were treated by Calyculin A (200 nM) at 37℃ for 18 hours after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
