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Phospho-MRE11 (Ser676) Polyclonal Antibody (CABP1514)

SKU:
CABP1514
$155
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Description

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Product Name:Phospho-MRE11 (Ser676) Polyclonal Antibody
SKU:CABP1514
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S676 of human MRE11 (NP_005582.1).
Sequence:IMSQ S
Tested Applications:WB
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:ATLD; HNGS1; MRE11A; MRE11B; Phospho-MRE11 (Ser676)
Positive Sample:HeLa+Calyculin A
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:81kDa
Observed MW:81kDa

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Purification Method:Affinity purification
Gene ID:4361
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from HeLa cells using Phospho-MRE11 (Ser676) Rabbit pAb (CABP1514) at 1:800 dilution. HeLa cells were treated by Calyculin A (200 nM) at 37℃ for 18 hours after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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