The Phospho-Met (Y1349) Polyclonal Antibody (CABP0077) is a crucial tool for researchers studying Met, a receptor tyrosine kinase involved in cell proliferation, survival, and migration. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting phosphorylation of the Y1349 site on Met in human samples, making it ideal for Western blot applications.Met, also known as hepatocyte growth factor receptor (HGFR), is a key player in cell signaling pathways that regulate processes like cell growth and tissue repair. The phosphorylation of specific tyrosine residues, such as Y1349, is essential for activating downstream signaling cascades that control cellular responses.
By targeting this phosphorylation site, researchers can gain insights into the role of Met in cancer progression, metastasis, and drug resistance.The Phospho-Met (Y1349) Polyclonal Antibody is a valuable tool for studying the phosphorylation status of Met in various cell types and tissues, providing essential information for cancer biology research and drug development. With its high reactivity and specificity, this antibody enables precise detection and quantification of phospho-Met (Y1349), facilitating in-depth analysis of Met signaling pathways and their implications in oncogenesis.
Product Name:
Phospho-MET-Y1349 Rabbit Polyclonal Antibody
SKU:
CABP0077
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic phosphorylated peptide around Y1349 of human MET (NP_000236.2).
Membrane, Secreted, Single-pass type I membrane protein.
Calculated MW:
156kDa
Observed MW:
160kDa
This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers.
Purification Method:
Affinity purification
Gene ID:
4233
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Phospho-MET-Y1349 antibody (CABP0077) at 1:1000 dilution. HeLa cells were treated by HGF (40ng/mL) for 10 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
