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Phospho-Met-Y1234/Y1235 Monoclonal Antibody (CABP1339)

SKU:
CABP1339
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
$167 - $731
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-Met-Y1234/Y1235 Monoclonal Antibody
SKU:CABP1339
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around Y1234 & Y1235 of human Met (NP_000236.2).
Sequence:KEYY SV
Tested Applications:WB ELISA
Recommended Dilution:WB,1:1000 - 1:5000
Synonyms:DA11; HGFR; AUTS9; RCCP2; c-Met; DFNB97; Phospho-Met-Y1234/Y1235
Positive Sample:HeLa+HGF,C2C12+HGF,C6+HGF
Conjugate:Unconjugated
Cellular Localization:basal plasma membrane, cell surface, extracellular region, plasma membrane
Calculated MW:156kDa
Observed MW:145kDa

This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers.

Purification Method:Affinity purification
Gene ID:4233
Clone Number:ARC54652
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using Phospho-Met-Y1234/Y1235 antibody (CABP1339) at1:2000 dilution.HeLa cells were treated by HGF (40 ng/ml) at 37℃ for 10 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.

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