The Phospho-Met (Y1234/1235) Rabbit Polyclonal Antibody (CABP0533) is a valuable tool for researchers studying the phosphorylation of the Met receptor tyrosine kinase. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the phosphorylated tyrosine residues Y1234 and Y1235 on the Met protein, this antibody enables precise detection and analysis in a variety of cell types.The Met receptor tyrosine kinase plays a crucial role in signaling pathways involved in cell growth, survival, and motility. Phosphorylation of specific tyrosine residues, such as Y1234 and Y1235, is known to regulate the activation and downstream signaling of Met, making this antibody essential for understanding the mechanisms by which Met activity is controlled.
Research into the phosphorylation status of the Met receptor can provide valuable insights into cancer biology, as dysregulation of Met signaling is commonly associated with tumorigenesis, metastasis, and drug resistance. By using the Phospho-Met (Y1234/1235) Rabbit Polyclonal Antibody in their studies, researchers can gain a better understanding of Met signaling pathways and potentially identify novel therapeutic targets for the treatment of cancer and other diseases.
Product Name:
Phospho-MET-Y1234/1235 Rabbit Polyclonal Antibody
SKU:
CABP0533
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around Y1234 & Y1235 of human MET (NP_000236.2).
Membrane, Secreted, Single-pass type I membrane protein.
Calculated MW:
156kDa
Observed MW:
145kDa
This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers.
Purification Method:
Affinity purification
Gene ID:
4233
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Phospho-MET-Y1234/1235 antibody (CABP0533) at 1:1000 dilution.HeLa cells were treated by HGF (40 ng/ml) at 37℃ for 10 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
