The Phospho-MEK1 (T292) Rabbit Monoclonal Antibody (CABP1021) is a high-quality antibody specifically designed for research involving the phosphorylation of MEK1 at threonine 292. This antibody, developed and validated for use in Western blot applications, is highly reactive with human samples.MEK1 is a crucial kinase in the MAPK signaling pathway, playing a key role in cell growth, proliferation, and differentiation. Phosphorylation of MEK1 at threonine 292 is known to regulate the activity of MEK1 and downstream signaling events.
This phosphorylation event is often dysregulated in cancer and other diseases, making it an important target for research.By using the Phospho-MEK1 (T292) Rabbit Monoclonal Antibody, researchers can accurately detect and analyze the phosphorylation status of MEK1 in various cell types, providing valuable insights into signaling pathways and potential therapeutic targets. This antibody is an essential tool for studies in cancer research, cell signaling, and drug development.
Product Name:
Phospho-MEK1-T292 Rabbit Monoclonal Antibody
SKU:
CABP1021
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T292 of human Phospho-MEK1-T292 (Q02750).
Cytoplasm, Membrane, Nucleus, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center, spindle pole body.
Calculated MW:
43kDa
Observed MW:
45kDa
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Purification Method:
Affinity purification
Gene ID:
5604
Clone Number:
ARC1566
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of C6 cells, using Phospho-MEK1-T292 Rabbit mAb (CABP1021) at 1:1000 dilution.C6 cells were treated by Nocodazole (50 ng/mL) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1min.
