The Phospho-MEK1-S298 Rabbit Monoclonal Antibody (CABP1020) is a highly specific antibody designed for research involving phosphorylated MEK1 at serine 298, a critical signaling molecule in the MAPK pathway. The antibody, raised in rabbits, is highly reactive with human samples and is validated for use in Western blot and immunohistochemistry applications. It specifically binds to the phosphorylated form of MEK1 at serine 298, enabling accurate detection and analysis in various cell types.MEK1 is a key kinase in the MAPK signaling pathway, playing a crucial role in cell proliferation, differentiation, and survival. Phosphorylation of MEK1 at serine 298 is known to modulate its activity, leading to downstream activation of ERK and other signaling molecules.
Research into the phosphorylation status of MEK1 can provide valuable insights into the regulation of cell signaling and its implications in various diseases, including cancer and developmental disorders.Understanding the phosphorylation of MEK1 at serine 298 is essential for unraveling the complexities of the MAPK signaling pathway and its role in disease pathogenesis. The Phospho-MEK1-S298 Rabbit Monoclonal Antibody (CABP1020) offers researchers a reliable tool for studying this important post-translational modification and its impact on cellular signaling processes.
Product Name:
Phospho-MEK1-S298 Rabbit Monoclonal Antibody
SKU:
CABP1020
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic phosphorylated peptide around S298 of human Phospho-MEK1-S298 (Q02750).
Sequence:
PLSS Y
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
MEL; Phospho-MEK1-S298
Positive Sample:
HeLa,C6,HeLa PMA,C6 PMA
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Membrane, Nucleus, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center, spindle pole body.
Calculated MW:
43kDa
Observed MW:
45kDa
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Purification Method:
Affinity purification
Gene ID:
5604
Clone Number:
ARC1565
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-MEK1-S298 Rabbit mAb (CABP1020) at 1:1000 dilution.Both HeLa cells and C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour or treated by PMA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1min.
