The Phospho-MEF2C (S396) Polyclonal Antibody (CABP0075) is a valuable tool for researchers studying MEF2C, a transcription factor involved in various cellular processes, including cell differentiation and survival. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it suitable for Western blot applications.MEF2C phosphorylation at serine 396 is known to regulate its activity, affecting gene expression and cell fate decisions. The CABP0075 antibody specifically targets this phosphorylation site, allowing for the detection and analysis of phospho-MEF2C levels in different cell types.
This feature makes it an ideal choice for investigations into signaling pathways, developmental biology, and neurological disorders related to MEF2C function.The phosphorylation of MEF2C at S396 has been linked to various diseases, such as neurodevelopmental disorders, cardiovascular diseases, and cancer. By utilizing this antibody, researchers can gain insights into the role of phospho-MEF2C in these pathologies, paving the way for the development of novel therapeutic strategies targeting this transcription factor.
Product Name:
Phospho-MEF2C-S396 Rabbit Polyclonal Antibody
SKU:
CABP0075
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S396 of human MEF2C (NP_002388.2).
This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe cognitive disability, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described.
Purification Method:
Affinity purification
Gene ID:
4208
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from K-562 and MCF-7 cells, using Phospho-MEF2C-S396 Rabbit pAb (CABP0075) at 1:1000 dilution. K562 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. MCF7 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
