The Phospho-MDM2 (S260) Polyclonal Antibody (CABP0074) is a valuable tool for researchers studying the phosphorylation of MDM2 at serine 260. MDM2, a key regulator of the p53 tumor suppressor protein, is involved in cell growth, apoptosis, and DNA repair. Phosphorylation at serine 260 plays a role in MDM2 activity and stability, making it a crucial modification to study in the context of cancer and other diseases.This antibody, raised in rabbits, has high reactivity with human samples and is validated for Western blot applications. It specifically binds to the phosphorylated form of MDM2 at serine 260, allowing for accurate detection and analysis in various cell types.
Its use is ideal for researchers interested in understanding the role of MDM2 phosphorylation in cancer biology, cell cycle regulation, and tumor development.By studying the phosphorylation of MDM2 at serine 260, researchers can gain insights into the complex regulatory pathways that influence cell growth and survival. This antibody is a valuable tool for advancing our understanding of MDM2 function and its potential as a therapeutic target in cancer treatment.
Product Name:
Phospho-MDM2-S260 Rabbit Polyclonal Antibody
SKU:
CABP0074
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S260 of human MDM2 (NP_001354919.1).
Sequence:
SLDS E
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
HDMX; LSKB; hdm2; ACTFS; Phospho-MDM2-S260
Positive Sample:
293
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus, nucleolus, nucleoplasm.
Calculated MW:
55kDa
Observed MW:
95kDa
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
Purification Method:
Affinity purification
Gene ID:
4193
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of 293 cell line, using phospho-MDM2-S260 antibody (CABP0074).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
