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Anti-Phospho-MDM2-S166 Antibody (CABP0073)

SKU:
CABP0073
Product Type:
Antibody
Applications:
WB
IF
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
  • Immunology Antibodies 3 Anti-Phospho-MDM2-S166 Antibody CABP0073
  • Immunology Antibodies 3 Anti-Phospho-MDM2-S166 Antibody CABP0073
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Description

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Anti-Phospho-MDM2-S166 Antibody (CABP0073)

The Phospho-MDM2 (S166) Polyclonal Antibody (CABP0073) is a valuable tool for researchers studying the MDM2 protein, a key regulator of the tumor suppressor protein p53. This antibody, produced in rabbits, is highly sensitive and specific to phosphorylated MDM2 at serine 166 in human samples, making it ideal for Western blot and immunohistochemistry applications.MDM2 is known for its role in regulating the stability and activity of p53, a crucial factor in cell cycle control and tumor suppression. Phosphorylation of MDM2 at serine 166 has been implicated in the modulation of its activity, leading to alterations in p53 signaling pathways and cellular responses to DNA damage and stress.

By using the Phospho-MDM2 (S166) Polyclonal Antibody, researchers can confidently analyze the phosphorylation status of MDM2 in various cell types and conditions, shedding light on the intricate regulatory mechanisms governing p53 function. This antibody is a valuable asset for studies in cancer biology, molecular biology, and drug development targeting the MDM2-p53 pathway.

Antibody Name:Anti-Phospho-MDM2-S166 Antibody
Antibody SKU:CABP0073
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic phosphorylated peptide around S166 of human MDM2 (NP_002383.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:1000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293
Immunogen:A synthetic phosphorylated peptide around S166 of human MDM2 (NP_002383.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TSSR R
Gene ID:4193
Uniprot:Q00987
Cellular Location:Cytoplasm, Nucleus, nucleolus, nucleoplasm
Calculated MW:11-14kDa/24-55kDa
Observed MW:95kDa
Anti-Phospho-MDM2-S166 Antibody (CABP0073)Western blot analysis of extracts of 293 cells, using Phospho-MDM2-S166 antibody (CABP0073). 293 cells were treated by Hydroxyurea (4mM) for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Anti-Phospho-MDM2-S166 Antibody (CABP0073)Immunofluorescence analysis of U2OS cells using Phospho-MDM2-S166 antibody (CABP0073). Blue: DAPI for nuclear staining.
Synonyms:MDM2, ACTFS, HDMX, hdm2
Background:This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
UniProt Protein Function:E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation (PubMed:12821780, PubMed:15053880, PubMed:15195100, PubMed:15632057, PubMed:16337594, PubMed:17290220, PubMed:19098711, PubMed:19219073, PubMed:19837670, PubMed:19965871, PubMed:20173098, PubMed:20385133, PubMed:20858735, PubMed:22128911). Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells. Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis.
NCBI Summary:This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013]
UniProt Code:Q00987
NCBI GenInfo Identifier:266516
NCBI Gene ID:4193
NCBI Accession:Q00987.1
UniProt Secondary Accession:Q00987,Q13226, Q13297, Q13298, Q13299, Q13300, Q13301 Q53XW0, Q71TW9, Q8WYJ1, A6NL51, A8K2S6,
UniProt Related Accession:Q00987
Molecular Weight:55,991 Da
NCBI Full Name:E3 ubiquitin-protein ligase Mdm2
NCBI Synonym Full Names:MDM2 proto-oncogene
NCBI Official Symbol:MDM2  
NCBI Official Synonym Symbols:HDMX; hdm2; ACTFS  
NCBI Protein Information:E3 ubiquitin-protein ligase Mdm2
UniProt Protein Name:E3 ubiquitin-protein ligase Mdm2
UniProt Synonym Protein Names:Double minute 2 protein; Hdm2; Oncoprotein Mdm2; RING-type E3 ubiquitin transferase Mdm2Curated; p53-binding protein Mdm2
Protein Family:Mdm2-binding protein
UniProt Gene Name:MDM2  

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