The Phospho-MCL1 (T163) Rabbit Polyclonal Antibody (CABP0943) is a valuable tool for researchers studying the MCL1 protein, a key regulator of cell survival and apoptosis. This antibody is highly specific for detecting phosphorylation of MCL1 at threonine 163, a post-translational modification that impacts its stability and anti-apoptotic function.Raised in rabbits, this antibody is validated for use in Western blot applications with human samples, allowing for reliable and accurate detection of phospho-MCL1 (T163) levels. Its ability to specifically recognize phosphorylated MCL1 makes it an essential tool for investigating the role of this protein in cancer and other diseases, as dysregulation of MCL1 is linked to tumorigenesis and therapeutic resistance.
By enabling precise detection and analysis of phospho-MCL1 (T163) in various cell types and experimental conditions, this antibody facilitates in-depth studies on the signaling pathways and mechanisms involving MCL1 phosphorylation. Researchers in the fields of oncology, cell biology, and drug development will benefit from using this antibody to explore the intricate functions of MCL1 and its potential as a target for therapeutic intervention.
Product Name:
Phospho-MCL1-T163 Rabbit Polyclonal Antibody
SKU:
CABP0943
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around T163 of human MCL1 (NP_068779.1).
This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing.
Purification Method:
Affinity purification
Gene ID:
4170
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-MCL1-T163 Rabbit pAb (CABP0943) at 1:1000 dilution.NIH/3T3 cells were treated by PMA (80 nM) for overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
