The Phospho-MARCKS (S162) Polyclonal Antibody (CABP0403) is a valuable tool for researchers studying MARCKS, a protein involved in regulating cell signaling pathways and membrane trafficking. This antibody, generated in rabbits, has high specificity for phosphorylated MARCKS at serine 162 in human samples, making it ideal for Western blot applications.MARCKS is a key player in cellular processes such as cell migration, adhesion, and neurite outgrowth, as well as in diseases like cancer and neurological disorders. The phosphorylation of MARCKS at serine 162 is known to impact its function, making this antibody essential for studying the role of MARCKS in cellular signaling and disease progression.
By targeting the phosphorylated form of MARCKS, researchers can gain insight into how this protein contributes to the development and progression of various diseases, paving the way for the development of targeted therapies and interventions. The Phospho-MARCKS (S162) Polyclonal Antibody is a valuable tool for researchers looking to deepen their understanding of MARCKS biology and its implications in human health.
Product Name:
Phospho-MARCKS-S162 Rabbit Polyclonal Antibody
SKU:
CABP0403
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A phospho specific peptide corresponding to residues surrounding S162 of human MARCKS
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Synonyms:
MACS; 80K-L; PKCSL; PRKCSL; Phospho-MARCKS-S162
Positive Sample:
293T
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Lipid-anchor, Membrane, cytoskeleton.
Calculated MW:
32kDa
Observed MW:
75kDa
The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.
Purification Method:
Affinity purification
Gene ID:
4082
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Phospho-MARCKS-S162 antibody (CABP0403) at 1:400 dilution.293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
