The Phospho-MAPT (T498) Polyclonal Antibody (CABP0895) is a valuable tool for researchers studying the MAPT protein, also known as Tau protein. This antibody, produced in rabbits, specifically targets the phosphorylated form of MAPT at threonine 498, a key site implicated in the regulation of Tau function and pathology in neurodegenerative diseases like Alzheimer's.With high reactivity towards human samples, this antibody is ideal for Western blot applications, enabling the detection and quantification of phospho-MAPT (T498) in various cell types and tissues.
Its specificity towards this phosphorylation site allows for detailed analysis of MAPT signaling pathways and potential therapeutic targets for neurodegenerative disorders.The phosphorylation of MAPT at T498 is known to affect its interactions with microtubules and other proteins, influencing its role in neuronal function and pathology. By utilizing this antibody in research, scientists can gain valuable insights into the mechanisms underlying Tau-related diseases and potentially identify new therapeutic strategies for treating these devastating conditions.
Product Name:
Phospho-Tau-T181 Rabbit Polyclonal Antibody
SKU:
CABP0895
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T498 of human Tau (NP_058519.3?).
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
Purification Method:
Affinity purification
Gene ID:
4137
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-Tau-T181 Rabbit pAb (CABP0895) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
