The Phospho-MAPT (S519/T522) Polyclonal Antibody (CABP0894) is specially designed for research involving MAPT, a protein associated with microtubule assembly and stabilization in neuronal cells. This antibody, generated in rabbits, is highly specific to phosphorylated MAPT at sites S519 and T522 in human samples, making it an excellent tool for studying the regulation of microtubule dynamics and neuronal function.Phosphorylation of MAPT at these specific sites has been linked to neurodegenerative diseases such as Alzheimer's and Parkinson's, making this antibody particularly valuable for researchers investigating the pathogenesis of these conditions. Its validation for use in Western blot applications allows for precise detection and quantification of phosphorylated MAPT levels in various experimental settings.
Furthermore, the ability of this antibody to specifically target phosphorylated MAPT provides insights into the role of this protein in neurological disorders and opens up new possibilities for developing targeted therapies aimed at modulating microtubule stability and neuronal health. Researchers in the fields of neuroscience and neurology will find this antibody invaluable for unraveling the intricate mechanisms underlying neurodegenerative diseases and discovering novel therapeutic strategies.
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
Purification Method:
Affinity purification
Gene ID:
4137
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Hela cells, using Phospho-Tau-Ser202/Thr205 Rabbit pAb (CABP0894) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
