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Phospho-MAPKAPK2-T334 Rabbit Polyclonal Antibody (CABP0588)

SKU:
CABP0588
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-MAPKAPK2-T334 Rabbit Polyclonal Antibody
SKU:CABP0588
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around T334 of human MAPKAPK2 (NP_116584.2).
Sequence:PQTP L
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:MK2; MK-2; MAPKAP-K2; Phospho-MAPKAPK-2/MK2-T334
Positive Sample:NIH/3T3,C6
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:46kDa
Observed MW:42kDa

This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:9261
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using Phospho-MAPKAPK-2/MK2-T334 antibody (CABP0588) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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