The Phospho-MAPKAPK2 (T334) Polyclonal Antibody (CABP0588) is a valuable tool for researchers studying the phosphorylation of MAPKAPK2 at threonine 334. MAPKAPK2 is a protein kinase involved in the MAPK signaling pathway, which plays a key role in cell proliferation, differentiation, and apoptosis. Phosphorylation of MAPKAPK2 at T334 is known to regulate its activity and substrate specificity.This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. It specifically binds to the phosphorylated form of MAPKAPK2 at T334, enabling researchers to detect and analyze the phosphorylation status of this protein in various cell types.
This makes it an ideal tool for studies in cell signaling, inflammation, and cancer research.Understanding the phosphorylation of MAPKAPK2 at T334 is important for unraveling the intricate signaling networks that control cellular responses to external stimuli. Dysregulation of MAPKAPK2 phosphorylation has been implicated in various diseases, including cancer and inflammatory disorders. By studying the phosphorylation status of MAPKAPK2, researchers can gain insights into the mechanisms underlying these diseases and potentially uncover new therapeutic targets for intervention.
Product Name:
Phospho-MAPKAPK2-T334 Rabbit Polyclonal Antibody
SKU:
CABP0588
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T334 of human MAPKAPK2 (NP_116584.2).
Sequence:
PQTP L
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
MK2; MK-2; MAPKAP-K2; Phospho-MAPKAPK-2/MK2-T334
Positive Sample:
NIH/3T3,C6
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
46kDa
Observed MW:
42kDa
This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
9261
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-MAPKAPK-2/MK2-T334 antibody (CABP0588) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
