The Phospho-MAPK3 (T202) Polyclonal Antibody (CABP0234) is a valuable tool for researchers studying the MAPK3 protein and its phosphorylation at threonine 202. MAPK3, also known as ERK1, is a crucial signaling molecule involved in various cellular processes, including cell growth, differentiation, and survival.This polyclonal antibody, generated using rabbits, exhibits high specificity towards the phosphorylated form of MAPK3 at threonine 202 in human samples. It has been validated for use in Western blot applications, allowing for the detection and analysis of phosphorylated MAPK3 in different cell types.
The phosphorylation of MAPK3 at threonine 202 plays a key role in regulating its activity and function in signal transduction pathways. Research into the phosphorylation status of MAPK3 is essential for understanding its role in various biological processes and diseases, such as cancer, where dysregulation of MAPK signaling pathways is common.Overall, the Phospho-MAPK3 (T202) Polyclonal Antibody is a valuable tool for researchers interested in studying the phosphorylation status of MAPK3 and its implications in cellular signaling and disease progression.
Product Name:
Phospho-ERK1-T202 Rabbit Polyclonal Antibody
SKU:
CABP0234
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T202 of human ERK1/ERK2 (NP_002737.2).
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
5594/5595
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-ERK1-T202 + ERK2-T185 Rabbit pAb (CABP0234) at 1:1000 dilution.Jurkat and NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Jurkat cells were treated by ATP(5 mM) at 30℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
