The Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Rabbit Monoclonal Antibody (CABP0974) is a powerful tool for researchers studying the phosphorylation of ERK1 and ERK2, key proteins in the MAPK signaling pathway. This antibody is specifically designed to detect phosphorylation at threonine 202 and tyrosine 204 in ERK1, and threonine 185 and tyrosine 187 in ERK2, making it a valuable resource for investigating the activation of these kinases in cell signaling pathways.The antibody, developed using rabbit monoclonal technology, offers high specificity and sensitivity for detecting phosphorylated ERK1 and ERK2 in a variety of cell types and experimental conditions.
Validated for use in Western blot applications, this antibody enables researchers to visualize and quantify the phosphorylation status of ERK1 and ERK2, providing insights into the regulation of cell proliferation, differentiation, and survival.With its versatility and reliability, the Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Rabbit Monoclonal Antibody (CABP0974) is an essential tool for studies in cell signaling, cancer research, and drug development. By targeting phosphorylated ERK1 and ERK2, researchers can gain a better understanding of the mechanisms underlying various physiological and pathological processes, paving the way for the
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
Purification Method:
Affinity purification
Gene ID:
5595/5594
Clone Number:
ARC1595
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (CABP0974) at 1:1000 dilution.Both 293T cells and NIH/3T3 cells were treated by ATP (5 mM) at 30℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
