The Phospho-MAPK14 (Y182) Polyclonal Antibody (CABP0057) is a valuable tool for researchers studying the phosphorylation of MAPK14, a protein involved in signaling pathways related to cell growth, differentiation, and inflammation. This antibody, produced in rabbits, is highly specific for the phosphorylated form of MAPK14 and has been validated for use in Western blot applications with human samples.MAPK14, also known as p38 MAP kinase, is a key regulator of cellular responses to stress and pro-inflammatory cytokines. Phosphorylation of MAPK14 at tyrosine 182 is crucial for its activation and downstream signaling cascade, making this antibody an essential tool for studying MAPK14 activity in various cellular contexts.
Researchers in the fields of cell biology, immunology, and cancer research will find this antibody particularly useful for investigating the role of MAPK14 phosphorylation in disease pathways and therapeutic development.By providing researchers with a reliable tool for detecting and analyzing phospho-MAPK14, the CABP0057 antibody opens up new possibilities for understanding the complex signaling networks controlled by MAP kinases. Its high specificity and sensitivity make it an essential component of any study involving MAPK14 phosphorylation and its implications for cellular function and disease progression.
Product Name:
Phospho-p38 MAPK-Y182 Rabbit Polyclonal Antibody
SKU:
CABP0057
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around Y182 of human MAPK (NP_620581.1).
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Purification Method:
Affinity purification
Gene ID:
1432
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Phospho-p38 MAPK-Y182 Rabbit pAb (CABP0057) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
